Assessing Salinity Tolerance in Rice Mutants by Phenotypic Evaluation Alongside Simple Sequence Repeat Analysis

Can Thu Huong,Tran Dang Xuan,Hoang-Dung Tran,Truong Thi Tu Anh,Vu Xuan Duong,Tran Dang Khanh,Nguyen Thanh Trung

doi:10.3390/agriculture10060191

Abstract

Salinity stress is one of the most severe constraints limiting rice production worldwide. Thus, the development of salt-tolerant rice promises to deal with increasing food demand due to climate change effects. This study investigated the salinity tolerance of mutant rice by evaluating phenotype and genotype, using forty-two simple sequence repeat (SSR) markers linked to the salinity tolerance Saltol quantitative trait locus (QTL) in ten cultivars and mutant lines. Results of phenotypic screening showed that the mutant line SKLo/BC15TB and cultivar BC15TB performed salt tolerance, while the mutant line Bao Thai/DT 84 and cultivar DT84DB were sensitive to salt stress. The markers RM 493, RM 562, RM 10748, RM 518, RM 237, and RM 20224 were the most polymorphic in salinity tolerance. Among them, RM 237, RM 10748, and RM 224 showed the highest polymorphism information (PIC = 0.58). This study reveals that the three markers are profitable for classification of salinity tolerance in both cultivar and mutant rice. The mutant line SKLo/BC15TB and cultivar BC15TB were found to be promising candidates for diversity analysis of salt-tolerant rice. Findings of this study are useful for developing new salinity-tolerant rice cultivars towards climate change.

Highlights

Rice is one of the most important crops grown and consumed globally
There was a classification of salt tolerance among 10 examined rice cultivars and lines
Negative values in root length reduction indicate that roots of these variety/mutant lines were elongated under saline treatment

Summary

Introduction

Rice is one of the most important crops grown and consumed globally. It ranks the third in agricultural production and provides daily meals for more than half of the world’s population.ongoing climate change adversely threatens rice production. Rice is one of the most important crops grown and consumed globally. It ranks the third in agricultural production and provides daily meals for more than half of the world’s population. Ongoing climate change adversely threatens rice production. Agriculture 2020, 10, 191 cause a significant yield loss in large rice-growing areas, including high salinity, drought, heat, and cold. Salinity stress is the main hazardous factor of rice productivity [1]. Rice is highly sensitive to salty conditions, especially at the seedling stage. The high concentration of toxic ions Na+ , induced by salinity, is the main cause of various physiological damages and inhibitory processes of plants.

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