Abstract
Nyamplung (Calophyllum inophyllum L) is known to be associated with AMF, but it is still unknown whether the association has begun to occur when cotyledons are still present or starting at a certain level of regeneration. Research has been done in Pecal Beach Kinjil River in Ketapang district. The purposes of the research are: (1) to assess the amount of spores and genus AMF associated with its development in the rhizosphere of nyamplung regeneration nature seedling stage from 10 cm to 150 cm, and (2) to determine root infection percentage in order to know the level of the association at any size of penage regeneration seedlings. The research is a field research by survey method with sampling technique against natural regeneration seedling level of 10 cm, 30 cm, 60 cm, 90 cm 120 cm, and 150 cm. The data was collectedin laboratoryon the form of the number of spores and genus AMF and the percentage of infections in the roots. The research results showed that there were 616 AMF spores fruit / 100 g of sandy soil consisting of the genus Glomus ( 597 pieces / 100 g sandy soil), Gigaspora ( 15 pieces / 100 g sandy soil) and Scutellospora ( 4 pieces / 100 g sandy soil). The development of the number of spores increased from the height of the seedling's natural regeneration measuring was 10 cm to 150 cm. The results of observations on the roots found thatAMF infection is in the form of vesicles and arbuscular. Infection at the root of natural penage regeneration from 84.44-100%, indicating the level of association between AMF and nyamplung seedling's natural regeneration in the high class ( grade 5 ) . Keywords: Arbuscular Mycorrhizal Fungi (AMF) Nyamplung (Calophyllum inophyllumL) Natural Seedling Regeneration, Pecal Beach
Highlights
Nyamplung (Calophyllum inophyllum L) is known to be associated with Arbuscular Mycorrhizal Fungi (AMF), but it is still unknown whether the association has begun to occur when cotyledons are still present or starting at a certain level of regeneration
Research has been done in Pecal Beach Kinjil River in Ketapang district
The purposes of the research are: (1) to assess the amount of spores and genus AMF associated with its development in the rhizosphere of nyamplung regeneration nature seedling stage from 10 cm to 150 cm, and (2) to determine root infection percentage in order to know the level of the association at any size of penage regeneration seedlings
Summary
Hasil penelitian berupa pengamatan dan identifikasi spora FMA dari 18 sampel pada rhizosfer akar permudaan alam nyamplungtingkat semai 10 cm, 30 cm, 60 cm, 90 cm, 120 cm dan 150 cm disajikan pada Tabel 1.Dari hasil penelitian tersebut dapat diketahui bahwa terdapat spora. Tabel 1 menunjukkan bahwa hasil tersebut semakin banyak dengan perhitungan spora FMA yang diambil bertambahnya ukuran tinggi permudaan dari saringan ukuran 125 μm dan 63 μm tingkat semai. Berdasarkan identifikasi menunjukan bahwa semakin tinggi sampai pada tingkat genus dalam Tabel ukuran tinggi permudaan alam. 2, pada rhizosfer permudaan tingkat nyamplung, jumlah spora FMA semakin semai nyamplung ditemukan spora bertambah banyak.Pada ukuran tinggi genusGlomus sp sebanyak cm hanya ditemukan sebanyak 325 buah/100 g tanah berpasir, Gigasphora buah/100 g/ tanah, namun meningkat sp buah/100 g tanah berpasir dan sampai 931 buah/100 g/tanah pada. Rata-rata jumlah spora setiap genus FMA pada permudaan alam nyamplung tingkat semai Bertambahnya jumlah spora dan meningkatnya ukuran tinggi permudaan alam ada hubunganya dengan hasil fotosintesis berupa karbohidrat yang dihasilkan oleh tanaman. Genus FMA yang terdapat pada rhizosfer permudaan alam tingkat semai nyamplung (The AMF genus found in the natural regeneration rhizosphere of penage seedling level)
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