Application of UV/vis spectrophotometry based on using least squares support vector machine and continuous wavelet transform methods for the simultaneous analysis of antibiotics drugs in tablet formulation: Comparison with HPLC method

Abstract

In this study, chemotremic methods with the spectrophotometric method was used for simultaneous determination of Trimethoprim (TMP) and Sulfamethoxazole (SMZ) in Co-trimoxazole tablet without the need separation steps. The proposed methods included the least squares support vector machine (LS-SVM) and continuous wavelet transform (CWT). Modeling of suggested methods was tested by synthetic mixtures containing different concentrations of TMP and SMZ. Root mean square error (RMSE) of the LS-SVM model was 0.471 and 0.147 for TMP and SMZ, respectively. On the other hand, in the CWT method, different families of wavelets were studied. The Daubechies (Db2) and Biortogonal (rbio2.2) wavelet families were selected to analyze TMP and SMZ, respectively. In this method, the mean recovery values related to the synthetic mixtures were 99.69 % and 98.15 % for TMP and SMZ, respectively. The obtained results from analyzing the real sample (Co-trimoxazole) by the proposed methods were compared to the high-performance liquid chromatography (HPLC) as a reference method. One-way ANOVA test at the 95 % confidence level was applied to the results. The data showed that there were no significant differences between the proposed and the reference methods.