Application of nanomaterials in plant regeneration of rice (Oryza sativa L.)

Abstract

In recent years, the application of nanomaterials has been investigated by various studies, especially in their biological and agricultural features. This research aimed to evaluate the influence of zinc oxide (ZnO) and titanium dioxide (TiO2) nanoparticles on the quality of the plant regeneration in rice (Oryza sativa L. cv. RD49). Mature seed explants were used for callus induction and plant regeneration cultured on the medium supplemented with different concentrations of ZnO or TiO2 nanoparticles. The high percentage of the callus induction was achieved on the medium supplemented with 5 mg.L-1 ZnO nanoparticles or 20 mg.L-1 TiO2 nanoparticles when compared with control treatment (without nanoparticles). Various concentrations of TiO2 nanoparticles were also found to increase the percentage of the plant regeneration more than ZnO nanoparticles. The highest percentage of plant regeneration was observed on the medium containing the concentration of 20 mg.L-1 TiO2 nanoparticles. The in vitro rooting of regenerated shoots was hardened and successfully transplanted to the field after 30 days of transplantation. The results suggested that ZnO and TiO2 nanomaterials may have potential applications in the in vitro plant regeneration.