Application of Factorial Design for the Optimization of Pleurotus Afin Ostreatus Production Using Grass As Substrate

Abstract

The organic residue from gardens, which is generally not used efficiently, has been the central focus of this research work. That is why the present work used the factorial methodology in the optimization of the production of Pleurotus afín ostreatus using an organic residue, with the primary objective of using this organic residue as a substrate, adjusting it through the balance of the C/N ratio, for the specific cultivation of Pleurotus. This is one of the edible mushroom species worldwide, known for its mycelial biomass and bioactive components. The methodology comprised several stages, including propagation of the fungus, generation of seeds using barley grains, inoculation with 5% inoculum in polypropylene bags containing four different types of substrates, with five replicates for each variant. The resulting production was measured, and the nutritional analysis of the fruiting bodies obtained from the substrate with the highest productivity was carried out. The results obtained in the parameters of responses in the production of Pleurotus afin ostreatus on an organic residue by the ANOVA method were (p<0.05) and the F-values for the models of factor A (type of substrate), factor B (type of crop) and factor AB (type of substrate and crop) were 6.78, 2.99 and 6.10, respectively. The nutritional composition of the fruiting bodies obtained from this substrate was determined. These fruiting bodies exhibited a considerable water content, which reached 75.44%. On the other hand, a low concentration of carbohydrates was observed, with a value of 15.86%, and a minimal amount of fats, barely 0.13%. It is important to note that there was a particularly notable protein content, which reached 8.09% in relation to the wet weight. Finally, it is concluded that the production values obtained in this research work indicate that the best substrate for the growth of the fungus Pleurotus afín ostreatus is the organic residue S3, whose biological efficiency is among the highest values recorded (37.849%) in the cultivation of this fungus. In addition, in the optimization by means of the factorial design, it was observed that in order to have a greater productive capacity of Pleurotus afín ostreatus, it is necessary to work with a ratio of 40.13C/N and with a type of sampling per day with a mass amount of 77g.