Application of detection of coagulation molecular markers in early diagnosis of cerebral infarction

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Objective To explore the clinical significance of combined detection of serum prothrombin fragment 1+ 2 (F1+ 2), beta-thromboboglobulin (β-TG), thrombomodulin (TM) and D-Dimer (D-D) in the early diagnosis of cerebral infarction. Methods The case group selected 125 cases from the patients who were diagnosed as cerebral infarction in neurology department of the Gaotang County People's Hospital from January 2015 to June 2017. The patients with cerebral infarction were grouped according to the sizes of head CT or MRI lesions. There were 42 cases in the large infarction group, 40 cases in the middle infarction group and 43 cases in the small infarction group. According to the National Institutes of Health (NIHSS) score on the degree of nerve function defect: there were 26 cases in the severe group, 55 cases in the moderate group and 44 cases in the mild group. Fifty cases of health check-up outpatient were selected as control group. The levels of F1+ 2, β-TG, TM and D-D were measured by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). A single factor variance analysis was used to compare the levels of coagulation molecules among different groups, and the values of each index in the early diagnosis of cerebral infarction were compared under the receiver operating characteristic (ROC) curve (AUC). Results The serum levels of F1+ 2, β-TG, TM and D-D were statistically significant in the coutrol group, small infarct group, middle infarct group and large infarct group (F=62.42, 43.26, 56.73, 22.34; P 0.05). The sensitivity and specificity of F1+ 2 were 78.51% and 86.73%. The sensitivity and specificity of β-TG were 82.62%, 85.74%. The sensitivity and specificity of TM were 92.84%, 86.22%. The sensitivity and specificity of D-D were 67.46%, 80.81%. The sensitivity and specificity of F1+ 2, β-TG, TM and D-D were 96.32%, 98.63%. The ROC curves of the single item of F1+ 2, β-TG, TM and D-D were 0.854, 0.823, 0.876, 0.781, and the AUC of TM curve was the largest. Conclusion The combined determination of serum F1+ 2, β-TG, TM and D-D levels can provide an experimental basis for early diagnosis of cerebral infarction. Key words: Brain infarction; Thrombin; Thrombosis; Biological markers; Early diagnosis