Abstract
Abstract Purpose To present results of transplantations of cultured corneal epithelium in limbal stem cell insufficiency. Methods 26 patients were donors of limbal epithelium for corneal epithelial culture. Patients suffered from limbal deficiency in one eye after chemical or thermal burn. Limbal cells from 2 mm2 biopsy were seeded on amniotic membrane. Cultures were carried in standard conditions in supplememted DMEM in presence of 3T3 fibroblasts. After superficial keratectomy amniotic carries with epithelial cells were transplanted on denuded corneas. Stabilisation of corneal surface was evaluated. Results 3 months after surgery 61,5% of eyes showed stabile epithelium with corneal slight haze caused by the amnion. In 50,0% of eyes there was no recurrent conjuntival neovascularisation. 38,4% of eyes remained cloudy due to stromal revascularization. In 4 eyes total conjuctival pannus developed again. Visual acuity ranged from counting fingers to 0,5. Conclusion Grafting of cultured epithelium is a promising method in treatment of limbal stem cell insufficiency in burns.
Published Version
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