Abstract
The purpose of the present study was to investigate the effect of local hydroxyapatite (HA) combined with extracted sea cucumber (Stichopus hermanni) collagen as a promising bone graft substitute on bone remodeling. Fourier-transform infrared spectroscopy, X-ray diffractometry, transmission electron microscopy, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and Sprague-Dawley rat model were used to characterize the microstructure, in vitro cytotoxicity, and in vivo bone-healing properties of the investigated biocomposite material. Analytical results found that the hydrothermal reaction-synthesized local HA had a hexagonal close-packed structure. The addition of extracted S. hermanni collagen did not influence the microstructure and functional groups of the local HA. Moreover, the MTT assay indicated that the investigated biocomposite material possessed a good in vitro biocompatibility. The in vivo animal study also revealed that the investigated biocomposite material exhibited the highest number of osteoblasts after 14 days of healing. Therefore, the results demonstrate that the local HA combined with extracted S. hermanni collagen could potentially enhance osteoblast formation in promoting bone healing and regeneration.
Highlights
Academic Editor: Elena Landi e purpose of the present study was to investigate the effect of local hydroxyapatite (HA) combined with extracted sea cucumber (Stichopus hermanni) collagen as a promising bone graft substitute on bone remodeling
The MTT assay indicated that the investigated biocomposite material possessed a good in vitro biocompatibility. e in vivo animal study revealed that the investigated biocomposite material exhibited the highest number of osteoblasts after 14 days of healing. erefore, the results demonstrate that the local HA combined with extracted S. hermanni collagen could potentially enhance osteoblast formation in promoting bone healing and regeneration
Introduction e alveolar bone gives major support to the immediate denture. e alveolar bone will experience resorption by 40% to 60% after tooth extraction, which will affect the use of immediate denture [1]. e denture is not stable if it is not supported by the alveolar bone [2]. e wound healing after tooth extraction is very important, especially before denture insertion [3]. e main problem encountered after tooth extraction is bone damage. e bone damage physiologically is resorption of the bone caused by tooth extraction [4]. e graft materials are applied to close gaps in the bone defect after tooth extraction resulting in bone formation, which allows the implant and denture to be installed [5, 6]
Summary
Academic Editor: Elena Landi e purpose of the present study was to investigate the effect of local hydroxyapatite (HA) combined with extracted sea cucumber (Stichopus hermanni) collagen as a promising bone graft substitute on bone remodeling. Erefore, the results demonstrate that the local HA combined with extracted S. hermanni collagen could potentially enhance osteoblast formation in promoting bone healing and regeneration. E alveolar bone will experience resorption by 40% to 60% after tooth extraction, which will affect the use of immediate denture [1]. E bone damage physiologically is resorption of the bone caused by tooth extraction [4]. E graft materials are applied to close gaps in the bone defect after tooth extraction resulting in bone formation, which allows the implant and denture to be installed [5, 6]. Parisi et al [14] have reported that incorporation of collagen from marine sponges into HA (especially the one mimicking the composition of the bone (with 70% of HA and 30% of spongin)) can improve the performance of the graft for bone regeneration applications
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call