Abstract
Bradykinin (Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg) (230 pmol) was dansylated with 5-N,N-dimethylaminonapthalenesulphonyl chloride (Dns-Cl), followed by isolation in a reversed-phase high-performance liquid chromatography system with fluorescence detection (emission 530 nm, excitation 350 nm(. The N-Dns-bradykinin was acid-hydrolysed and 1/500 of the liberated N-Dns-arginine was analysed on Hypersil ODS by gradient elution microbore high-performance liquid chromatography (5 μm, 150 × 2.1 mm I.D.) and postcolumn reaction with bis-(2,4,6-trichlorophenyl) oxalate0hydrogen peroxide. The generated chemiluminescence was detected by a photomultiplier. The technical porblems of achieving sensitive detection by this procedure are discussed.
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