ANTIPROLIFERATIVE AND ANTIOXIDANT EFFECTS OF CARNOSIC ACID ON HUMAN LIVER CANCER CELLS

Abstract

The purpose of the study was to investigate the cytotoxic effects of carnosic acid alone and in combination with cisplatin on human liver cancer cells and their capacity to scavenge reactive oxygen species induced in the presence or absence of hydrogen peroxide.Cytotoxic effects of agents on human liver cancer cells for 24 and 48 hours were evaluated by methyl-thiazol tetrazolium-bromide assay. Mitochondrial membrane potential were detected JC-1 kit. The intracellular reactive oxygen species levels were determined using 2’-7’dichlorofluorescin diacetateassay. According to our findings, both carnosic acid alone and in combination with cisplatin showed cytotoxic effects in human liver cancer cells at 24 and 48 hours of exposure. In particular, it was seen that the cell viability significantly decreased in a dose-dependent manner at 48 hours of exposure, and the combined treatment was found to have a more pronounced cytotoxic effect. In addition, all carnosic acid concentrations alone and in combination with cisplatin were identified to significantly reduce mitochondrial membrane potential. We observed that both carnosic acid alone and in combination with cisplatin lowered intracellular reactive oxygen species levels in the presence or absence of hydrogen peroxide. The results suggested that carnosic acid alone or in combination with cisplatin might be a promising agent in the treatment of liver cancer.