Abstract
The objective of this study was to evaluate the in vitro multiplication of Eremanthus incanus axillary buds in the presence of PVP additives and activated charcoal under the interaction of different sucrose and agar concentrations. Explants containing axillary buds were inoculated in MS culture medium to evaluate the effect of 0.8 g L -1 of PVP and 1.0, 2.0 and 4.0 g L -1 of activated charcoal. The results of the addition of 0, 15, 30, and 60 g L -1 of sucrose combined with concentrations of 6 and 10 g L -1 of agar in MS medium were also evaluated. The number and quality of emitted shoots were evaluated after 30 and 60 days. The addition of PVP to the culture medium was more efficient than the use of activated charcoal in the multiplication of the species. The presence of sucrose is indispensable for developing the shoots well, and the use of a lower concentration of agar favors the development of quality shoots. The best results for shoot multiplication were obtained using 30 g L -1 sucrose and 6 g L -1 agar. PVP, activated charcoal, agar and sucrose are important components in the culture medium and alter the in vitro multiplication of Eremanthus incanus .
Highlights
Eremanthus incanus (Less.) Less is a forest species popularly known as Candeia and has great importance for Brazilian biodiversity, from which high-value products used by the cosmetic and pharmaceutical industries such as essential oil and α-bisabolol are extracted, and they are widely used as fence posts for presenting highdurability wood (SCOLFORO et al, 2012)
In the control of oxidation by phenolic compounds, antioxidants such as PVP and activated charcoal can be included in the culture medium as an alternative to the problem
Activated charcoal is obtained from the thermal decomposition of carbon-rich materials, and in nutrient medium represents a thin network of pores with large surface and volume, which gives it a distinct adsorption capacity (THOMAS, 2008)
Summary
Eremanthus incanus (Less.) Less is a forest species popularly known as Candeia and has great importance for Brazilian biodiversity, from which high-value products used by the cosmetic and pharmaceutical industries such as essential oil and α-bisabolol are extracted, and they are widely used as fence posts for presenting highdurability wood (SCOLFORO et al, 2012).As this species has a great economic, ecological and social value (SCOLFORO et al, 2012), it is important to have greater attention and research performed regarding its propagation, aiming at improving multiplication techniques for possible applications in homogeneous plantations, recovery of degraded areas, genetic improvement and conservation of germplasm.FLORESTA, Curitiba, PR, v. 48, n. 3, p. 311-320, jul/set. 2018.Miranda.
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