Abstract
In vitro propagation increases the supply and commercialisation of products of interest. For this, optimising the growing conditions and the composition of the culture medium is crucial to benefit the full development of the plants. Thus, the objective was to evaluate the in vitro propagation of Aloysia triphylla on different culture media, with varying agar and sucrose concentrations. The experiment was conducted as a completely randomised design, 3×3×3 factorial scheme, with three culture media (MS, JADS and WPM), three sucrose concentrations (8, 10 and 12 g L-1) and three agar concentrations (15, 30 and 45 g L-1), with three replicates each and experimental units composed of one plant per replicate. After 25 days of cultivation, the fresh and dry mass of the plants, numbers of leaves, numbers of nodes, plant lengths, numbers of oxidised leaves, hyperhydricity and acclimatization percentages were evaluated. The WPM medium resulted in a reduced fresh mass, reflecting in the low hyperhydricity observed in the explants, and favoured the acclimatization of the plants. Thus, the WPM medium with sucrose (15 g L-1) and agar (12 g L-1) is recommended as the medium most suitable for the in vitro regeneration of Aloysia triphylla.
Highlights
Brazil stands out in the production of essential oils alongside India, China and Indonesia, which are considered the world's largest producers (Paulus, Valmorbida, Toffoli, & Paulus, 2014)
The fresh mass of plants showed a significant difference for the factors culture media × sucrose concentrations and sucrose concentrations × agar concentrations
Comparing sucrose concentrations × culture media, there was a significant difference only at the 15 and 30 g L-1 concentration, in which the MS medium was superior to JADS and WPM (Figure 1A)
Summary
Brazil stands out in the production of essential oils alongside India, China and Indonesia, which are considered the world's largest producers (Paulus, Valmorbida, Toffoli, & Paulus, 2014). The most important medicinal product of this plant is the essential oil, which contains neral, geraniol, limonene, cineol, β-caryophyllene and espatulenol, and it is responsible for a series of biological activities, such as antimicrobial, insecticidal, neurophysiological, anxiolytic, gastrointestinal, anti-inflammatory, cardiovascular and anticancer effects, among others (Gomes et al, 2009; Jimenez-Ferrer et al, 2017; Bahramsoltani et al, 2018). This essential oil has a high potential to be used as an alternative to the use of synthetic antibiotics (Souza et al, 2017) as well as anaesthetics, and it is capable of inducing aversive behaviour in fish (Junior et al, 2018). Current studies on the effectiveness of this plant are mainly related to antioxidant activity, with the improvement of the antioxidant capacity of blood cells, the induction of endogenous defence antioxidants and the reduction of oxidative damages (Bahramsoltani et al, 2018)
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