Abstract
Background Obesity and chronic ingestion of lipid-rich meals are related to an enhanced oxidative stress (OS). Aims To examine the influence of duodenal-jejunal omega switch surgery in combination with different diets on the antioxidative status in the soleus muscle of rats. Methods After 8 weeks on a high-fat diet (HF) or control diet (CD), rats underwent duodenal-jejunal omega switch (DJOS) or SHAM (control) surgery. After surgery, for the next 8 weeks, half of DJOS/SHAM animals were kept on the same diet as before, and half had a changed diet. The total superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR) activity as well as malondialdehyde (MDA) concentration were measured in the soleus of rats. Results CAT and GPx activity were significantly lower after DJOS surgery versus SHAM, regardless of the type of diet. The activity of CAT, SOD, GR, CuZnSOD, and GPx was altered in the CD/HF or HF/CD groups. After DJOS, the lowest muscle concentration of MDA was observed in the CD/CD group and the highest in CD/HF. Conclusions DJOS surgery significantly decreases the antioxidative system in soleus muscles of rats. CD/HF and HF/CD dietary patterns lead to an increase in antioxidative activity, while remaining on unchanged diet (CD or HF) is associated with a reduced oxidative stress.
Highlights
Obesity and comorbidities related to it pose a major challenge to public health systems and affected individuals worldwide
Column one presents a comparison between duodenal-jejunal omega switch (DJOS) and SHAM surgery associated with different diets, column two shows comparisons between dietary groups of DJOS-operated animals, and column three shows comparisons between dietary groups of SHAM-operated animals
In the DJOS surgery group, glutathione reductase (GR) muscle’s activity in the high-fat diet (HF)/control diet (CD) and CD/CD diet groups were significantly higher when compared to the SHAM-operated HF/CD and CD/CD diet groups of animals
Summary
Obesity and comorbidities related to it pose a major challenge to public health systems and affected individuals worldwide. Scientists are trying to understand the influence of various aspects related to different treatment methods like diets or metabolic surgery procedures on humans using animal models. In relation to HF diet, Auberval et al proved the increase of tissue but not plasma oxidative stress among the studied animals [12] and Pinho et al revealed that HFD induces skeletal muscle OS in rats [13]. We aimed to assess the influence of duodenal-jejunal omega switch (DJOS) surgery in combination with different types of diet on antioxidant status in the soleus skeletal muscles of rats, which to our best knowledge have not been studied up to date, neither in humans nor in animals. To examine the influence of duodenal-jejunal omega switch surgery in combination with different diets on the antioxidative status in the soleus muscle of rats. CD/HF and HF/CD dietary patterns lead to an increase in antioxidative activity, while remaining on unchanged diet (CD or HF) is associated with a reduced oxidative stress
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call