Abstract
Antioxidants have become OTC (Over the Counter) prophylactic dietary supplements hit of the 21st century. Polyphenols, including flavonoids and phenolic acids, are widely distributed antioxidants and are present in medicinal drugs, fruits and vegetables (food) e.g. propolis, wine, chocolate….Individuals tend to reach for polyphenols as a source of longevity which can be related to their ability to reduce oxidative stress. Oxidative stress has been postulated as a cause of many diseases e.g. rheumatoid arthritis, diabetes, skin diseases, cataract, Alzheimer and Parkinson disease… Question that we often face is how effective commercially available product as antioxidants is. Predominantly this answer is gained through in vitro testing that includes spectrophotometry and chromatography.
Highlights
Antioxidants have become OTC (Over the Counter) prophylactic dietary supplements hit of the 21st century
Individuals tend to reach for polyphenols as a source of longevity which can be related to their ability to reduce oxidative stress
While DPPH comes in radical form, ABTS has to be activated with potassium persulphate
Summary
Antioxidants have become OTC (Over the Counter) prophylactic dietary supplements hit of the 21st century. Polyphenols, including flavonoids and phenolic acids, are widely distributed antioxidants and are present in medicinal drugs, fruits and vegetables (food) e.g. propolis, wine, chocolate... Spectrophotometry methods of choice determine quantity of polyphenols or their antioxidant potential. The Folin-Ciocalteu method is used for quantification of total polyphenols and is based on the reduction of MoO4+ to MoO3+ that can be monitored at 765 nm as change in color from yellow to blue.
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