Abstract
The present study aimed to evaluate the in-vitro and in-vivo antitumor potential of Ipomoea pes-caprae (I. pes-caprae). Petroleum ether (60-80oC), methanol, aqueous, and swaras extracts prepared from the whole plant of I. pes-caprae. Antioxidant activity of I. pes-caprae was evaluated by using 2, 2-diphenyl-1-picrylhydrazylhydrate (DPPH), reducing power, and hydroxyl ion scavenging assay methods. The cytotoxic effect of I. pes-caprae was evaluated by using Trypan blue and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against animal melanoma cancer (B16F10), human stomach cancer (Kato-III), and human colorectal cancer (HT-29) cell lines. The ultraviolet (UV), infrared (IR), nuclear magnetic resonance (NMR), and Mass spectra were recorded for the structure elucidation of isolated compounds. These isolated compounds of I. pes-caprae were evaluated biologically at two doses of 25 and 12.5 mg/kg body weight on male C57BL mice to explore the antitumor activity against melanoma cancer. The scavenging range (IC50) of the extracts of I. pes-caprae was found to be 63.40±2.26 to 74.77±2.28 μg/ml, and the cytotoxic range (CTC50) for MTT assay was found to be 62.54±3.27 to 73.70±1.32 μg/ml. Based on IR, NMR, and mass spectroscopic analysis and by chemical transformation, structures of three compounds A, B, and C were elucidated as 3’-methoxy-3,4’,5,7-tetrahydroxy flavone, 3,7-dimethoxy-8-methyl-4’,5-dihydroxy flavone, and 3’-methoxy-4’,5,7-trihydroxy flavone-3-glucoside, respectively. All isolated compounds were shown antitumor potential against melanoma cancer.
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