Abstract
BackgroundPersicaria hydropiper (Linn.) Delarbre is a common plant of Polygonaceae family commonly called Bishkatali in Bangladesh. Leaves of the plant are traditionally used in the treatment of rheumatic pain, gout, and skin diseases such as ringworms, scabies, boils, abscesses, carbuncles, bites of snakes, dogs or insects. This study evaluated the antinociceptive effect of the methanol extract of P. hydropiper leaves (MEPH).MethodsThe antinociceptive activity of MEPH was investigated using heat-induced (hot-plate and tail-immersion test) and chemical-induced (acetic acid, formalin, glutamic acid, cinnamaldehyde) nociception models in mice at 25, 50, and 75 mg/kg doses. Involvement of opioid system, cyclic guanosine monophosphate (cGMP) pathway, and ATP-sensitive K+ channel pathway were also tested using naloxone, methylene blue and glibenclamide respectively.ResultsMEPH showed antinociceptive activity in both heat- and chemical induced pain models. In both hot plate and tail immersion tests MEPH significantly increases the latency to the thermal stimuli. In acetic acid-induced writhing test the extract inhibited the number of abdominal writhing. Likewise, MEPH produced significant dose-dependent inhibition of paw licking in both neurogenic and inflammatory pain induced by intraplantar injection of formalin. Besides, MEPH also significantly inhibited the glutamate-induced pain and cinnamaldehyde-induced pain in mice. It was also clear that pretreatment with naloxone significantly reversed the antinociception produced by MEPH in hot plate and tail immersion test suggesting the involvement of opioid system in its effect. In addition, administration of methylene blue, a non specific inhibitor of NO/guanylyl cyclase, enhanced MEPH induced antinociception while glibenclamide, an ATP-sensitive K+ channel antagonist, could not reverse antinociceptive activity induced by MEPH.ConclusionBased on the results of the current study it can be said that MEPH possesses significant antinociceptive activity which acts in both peripheral and central mechanisms.
Highlights
Persicaria hydropiper (Linn.) Delarbre is a common plant of Polygonaceae family commonly called Bishkatali in Bangladesh
Phytochemical screening The preliminary screening revealed the presence of alkaloid, carbohydrate, glycoside, steroid, flavonoid, saponin and tannin in MEPH
Involvement of cyclic guanosine monophosphate pathway The present study evaluated the effect of MEPH at 25, 50, and 75 mg/kg doses and methylene blue (20 mg/kg) treatments
Summary
Persicaria hydropiper (Linn.) Delarbre is a common plant of Polygonaceae family commonly called Bishkatali in Bangladesh. Leaves of the plant are traditionally used in the treatment of rheumatic pain, gout, and skin diseases such as ringworms, scabies, boils, abscesses, carbuncles, bites of snakes, dogs or insects. This study evaluated the antinociceptive effect of the methanol extract of P. hydropiper leaves (MEPH). Persicaria hydropiper (Linn.) Delarbre is a common plant of Polygonaceae family locally known as Bishkatali in Bangladesh. In the traditional medicine leaves are used to treat rheumatic pain, knee pain, gout, skin diseases such as ringworms, scabies, boils, abscesses, carbuncles, bites of snakes, dogs or insects, colic pain, diuretic, and amenorrhea. A number of bioactive constituents have been reported from various Persicaria species with anticancer, antioxidant, antinociceptive, antileukemic, antimicrobial and tyrosinase-inhibitory activities [6]. The plant is used to treat diarrhoea, dyspepsia, itching skin, excessive menstrual bleeding and hemorrhoids either alone or with other herbs [8]
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