Antimycobacterial activity and phytochemical properties of Eucalyptus camaldulensis (eucalyptus) extracted by deep eutectic solvents

Abstract

The tuberculosis (TB) is one of the world's top causes of illness and death, it is a major public health problem. Eucalyptus eucalyptus camaldulensis has been traditionally used against many diseases, such as respiratory disease infections, including pulmonary (TB),The investigate the antimycobacterial activity of extracts by different deep eutectic solvents against multidrug-resistant (MDR) Mycobacterium tuberculosis compared with aqueous traditional solvents and the correlation between the phytochemical composition and antimycobacterial activity, The deep eutectic solvents (DESs) and standard water solvents were used to extract the samples. Agar disc diffusion experiment was used to investigate the antimycobacterial activity of extracts against multidrug-resistant (MDR) Mycobacterium tuberculosis. The minimal inhibitory was determined spectrophotometrically at 570 nm and followed measured the bactericidal concentrations. Phytochemical analysis was used in the study to investigate the total phenolic and flavonoid concentrations (TPC and TFC), the demonstrated, extraction by DESs is more a beneficial procedure than traditional solvent extraction of camaldulensis extracts, which have remarkable effectiveness against tuberculosis activities. Therefore, the use of DES should be considered for further investigation.