Abstract
Aim. To determine correlation between the storage conditions and the activity of complex from Erwinia carotovora ОНУ320 carotovoricnes and Lactobacillus plantarum ONU87 active against phytopathogenic erwinias. Materials and methods. The complex consists of carotovoricines (defective phage particles) from the strain E. carotovora ОНУ320, obtained by spontaneous induction, and of bacteria L. plantarum ONU87 (cells titer – 105 CFU/ml) in 1:1. The complex and each component separately were stored for 31 days at -20, 4 and 20 °C. Stability control of the individual components of the mixture (bacteriocins killer activity and lactobacilli cells titer determination) was performed during all term of storage. Carotovoricines killer activity was determined according to size and diameter of lysing zones. L. plantarum ОНУ87 viability was determined as titer after the growth on MRS agar. Results. For the first time the optimal storage conditions for the mixture of L. plantarum ONU87 live cells and E. carotovora ОНУ320 carotovoricines were determibed. The negative influence of L. plantarum ONU87 strain on the activity of carotovoricine mixture component was shown, if it was stored at temperature 20 and 4 °C. Conclusions. The decrease of carotovoricines activity after the storage at 4 and 20 °С with lactobacilli possibly occurs because of destabilization of proteins that form defective phage tails and theirs selfcontraction.
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