STUDY OF THE EFFECT OF BACTERIA OF THE GENUS BACILLUS ON THE CULTURE OF RICE AND CORN SEEDS IN VITRO

Abstract

The aim of the study was to investigate the effect of bacteria strains Bacillus megaterium ONU500, Bacillus velezensis ONU553, Bacillus pumilus ONU554, Bacillus subtilis ONU559 on the germination and growth of rice and corn seeds under sterile conditions. Materials and methods. Three-day cultures of the four strains were grown on LB liquid nutrient medium in a thermostat at 28 °C and the seeds of model plants were sterilized according to the standard scheme. The seeds were placed in 1% solutions of bacterial suspensions (106 CFU/ml) of each strain and inoculated for 1 hour. Results. The experiment results on both model plants indicate significant potential of the bacteria B. megaterium ONU500, B. velezensis ONU553, B. pumilus ONU554 as growth and development stimulators for plants. B. subtilis ONU559 is recommended for further study and clarification of its impact on plants in subsequent research. Conclusion. The most positive, stimulating effect on the indicators of germination, growth, and development of rice and corn seeds is exerted by inoculation with the strain B. megaterium ONU500 (for rice: increase in shoot length by 36.5%, root by 29%; for corn: 50% and 69% respectively). Further study of the influence of B. velezensis ONU553 is promising for rice cultivation, and for corn – B. pumilus ONU554.