Abstract
One amber mutant, named sus50, of phage P1 with decreased abortive transduction was isolated from P1CM cts-1, a thermo-inducible mutant of PlCM, after hydroxylamine mutagenesis. When sus50 phage is grown in Su − host, defective phage particles are produced at a rate 670 times that of infective phage particles and, in addition, many head particles containing sus50 DNA and many phage tails are also produced. In generalized transduction by sus50 mutant phage, the frequency of abortive transduction per infective phage decreased to about one-tenth that of P1CM cts-1 sus + phage, while that of stable transduction appeared to be about 10 times higher than that of P1CM cts-1 sus + phage. Stimulation of stable transduction by uv irradiation was not observed. From revertant analysis of the sus50 mutant, it is suggested that sus50 might be a single mutation, forming defective phage particles as well as decreasing abortive transduction.
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