Abstract
Integration deficient ( L) mutants of phage P22 can be complemented with L + phage to yield L mutant lysogens. Once established the L prophage is stable in the absence of L + function. Ultraviolet or thermal induction of L lysogens leads to production of defective phage particles and low yields of infectious phage. The defective particles contain a normal amount of DNA but the DNA is mainly bacterial in origin and appears to derive from the pro C region adjacent to one end of the prophage. The defective lysates transduce pro C at high frequency relative to other bacterial markers. The representation of phage genes is strongly polar with markers distal to pro C being rarely present. It appears that L + function is required not only for efficient integration of the prophage, but for normal recovery of the prophage genome following induction.
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