Abstract
<p><em>Clostridium difficile</em> is a Gram-positive, anaerobic rod-shaped bacterium, which is a primary cause of nosocomial diarrhoea. The rising incidence of antibiotic resistance in pathogens such as <em>C. difficile</em> makes the new antibacterial natural products research very important and necessary to conduct. It has been observed that propolis has bactericidal effects<em>.</em> Propolis is a natural resinous product that is manufactured by honeybees (<em>Apis mellifera</em>). The antimicrobial activity of propolis is an important biological property. In spite of the large composition differences of the propolis types, all of them have antimicrobial activity. Twenty strains of the <em>C.difficile</em> belonging to four prominent PCR-ribotypes (RT) (RT017, RT023, RT027, and RT046) were used in research. MBC value were determined by broth dilution method. Propolis samples were obtained from honey bees farm in lubelskie woyevodship. Bactericidal effect of the propolis on <em>C.difficile</em> strains was observed at its concentration of 39 mg/ml.</p>
Highlights
S Clostridium dificile is a Gram-positive, strictly anaerobic, spore forming bacteria and the major cause of nosocomial diarrhea
Main virulence factors of C. dificile are the high molecular weight toxins: A (TcdA); 308kDa and B (TcdB); 270 kDa, which are essential for the disease manifestations
The aim of this study is to explore effects of propolis produced in Poland on the C. dificile strains belonging to the different ribotypes
Summary
Clostridium dificile is a Gram-positive, anaerobic bacilli which is a primary cause of nosocomial diarrhoea. It has been observed that propolis has bactericidal effects. Twenty strains of the C. dificile belonging to four prominent PCR-ribotypes (RT) (RT017, RT023, RT027, and RT046) were used in research. MBC value were determined by broth dilution method. Bactericidal effect of propolis on C. dificile strains was observed at 3.9 mg/ml concentration. Clostridium dificile gram dodatnia beztlenowa laseczka powodująca zakażenia szpitalne, jest zaliczana współcześnie do grupy najbardziej opornych na antybiotyki drobnoustrojów. Naturalnym środkiem biologicznie czynnym, wykazującym działanie przeciwdrobnoustrojowe jest produkt pszczeli propolis, MIChAŁ PIOTROWSKI, hANNA PITUCh, PIOTR OBUCh-WOSzCzATYńSKI substancja żywiczna wywarzana przez pszczoły miodne (Apis mellifera). Wartości MBC określono, wykorzystując metodę seryjnych rozcieńczeń w podłożu płynnym. Propolis wykazał działanie bakteriobójcze wobec badanych szczepów C. dificile przy stężeniu 3,9 mg/mL. Słowa kluczowe: propolis, minimalne stężenie bakteriobójcze, Clostridium dificile
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