Abstract
본 연구에서는 브로콜리 품종 및 부위에 따른 항균활성 및 항산화 효과를 조사하였다. 브로콜리 11개 품종에 대해 그람 양성균 Staphylococcus aureus, Listeria monocytogenes 2종과 그람 음성균 Escherichia coli O157:H7, Salmonella enteritidis 2종에 대한 항균활성을 조사한 결과, S. aureus에 대해 AMG 품종의 화뢰 추출물이 12.83mm, 잎 추출물 13.00 mm로 가장 높은 항균활성을 보였으나 부위별로는 큰 차이를 보이지 않았다. L. monocytogenes의 경우, 화뢰 추출물은 NJ 품종이 13.58mm, 잎 추출물은 YDR 품종이 13.92mm로 가장 큰 항균활성을 보였으며, 저해환의 평균값이 화뢰는 12.85mm, 잎은 13.17mm로 다른 4종의 균 중에서 가장 높은 값을 보였다. E. coli O157:H7에 대해서는 Grd 품종의 화뢰와 잎 추출물 모두 가장 높은 항균활성을 보였으나, 부위별로는 차이가 나타나지 않았으며, S. enteritidis는 화뢰추출물은 NJ 품종이 12.67mm, 잎 추출물은 NY, 0c 품종이 12.75mm의 저해환을 보였으며, 잎 추출물이 화뢰 추출물에 비해 높은 항균활성을 보여주었다. 품종별 항산화 효과를 살펴보면, 화뢰 추출물은 NJ 품종이 39.90%, 잎 추출물은 0c 품종이 43.64%로 가장 높았으며, 부위별 평균값은 화뢰추출물 23.35%, 잎 추출물 35.91%를 나타내어 잎 추출물의 항산화 효과가 약 1.5배 높게 나타났다. NJ 품종을 제외하고 모든 품종에서 잎 추출물의 전자공여능이 화뢰 추출물보다 높은 값을 나타내었다. The antimicrobial and antioxidant activities of different cultivars and parts of broccoli were investigated. The screening of antimicrobial activities for the floret and leaf extracts of 11 cultivars against selected gram positive (Staphylococcus aureus and Listeria monocytogenes) and gram negative bacteria (Escherichia coli O157:H7 and Salmonella enteritidis) were conducted. The AMG cultivar showed the highest antimicrobial activity against S. aureus with the floret and leaf extracts (12.83 and 13.00 mm). Antibacterial activity against L. monocytogenes was strongest with floret extract of NJ cultivar (13.58 mm) and leaf extract of YDR cultivar (13.92 mm). Moreover, the size of inhibition zone against L. monocytogenes was bigger than those of 4 kinds of pathogenic bacteria. Both floret and leaf extracts of Grd cultivar showed the highest antimicrobial activity against E. coli O157: H7, but there was no difference between floret and leaf extracts. Floret extract o f NJ cultivar and leaf extract of NY and 0c cultivars were effective against S. enteritidis whereas leaf extract exhibited better antimicrobial effect than floret extract. These results showed that floret extract of NJ and leaf extract of 0c had the highest antioxidant activity which was 39.90 and 43.64%, respectively. The antioxidant activity of leaf extract was 1.5 times higher than that of floret extract. All cultivars except NJ showed that electron donating ability of leaf extract was higher than that of floret extract.
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