In vitro Antioxidant and Antibacterial Activities of Jatropha heynei Leaf Extract and GC-MS Profiling

Abstract

Aim: Jatropha heynei N. P. Balakr. of family Euphorbiaceae is an endemic herb with ethno medicinal values has not been studied for phytochemical analysis. The present study investigates the antibacterial, antioxidant activity and GC-MS profiling of J. heynei leaf extract. Materials and Methods: The cyclic voltammetry technique was employed to study the antioxidant activity of leaf extract. In vitro antibacterial activity was done by agar well diffusion assay. Further, Chemical profiling of methanolic leaf extract of J. heynei was done by the GC-MS analysis. Results: Cyclic voltammograms of extract revealed two anodic peaks at 0.12 and 0.22V when anodic current was applied at scan rate of 50 mV s-1. This low oxidation peak potential in the extract suggested its good antioxidant property. In vitro antibacterial activity extract showed good inhibitory effect against Staphylococcus aureus with zone of inhibition 14±0.57 mm and Pseudomonas aeruginosa with zone of inhibition 10±0.25mm. GC-MS analysis of leaf extract revealed presence of 14 prominent chemical compounds. Among them, major abundant compounds includes pentadecanoic acid, phytol, cis, cis, cis-7, 10, 13-hexadecatrienal Hexadecanoic acid, 2-hydroxy- 1-(hydroxymethyl) ethyl ester, γ-Sitosterol and Lupeol. Five compounds such as D-chiro-Inositol 3-O-(2-amino-4-((carboxyiminomethyl) amino)-2, 3, 4, 6-tetradeoxy-.alp, phytol, cis, cis, cis-7, 10, 13-hexadecatrienal, vitamin E acetate and β-sitosterol were associated with antioxidant property and compounds like 1, 2, 3-propanetriol monoacetate, octadecanoic acid, phytol, 2,6,10,14,18,22-Tetracosahexaene, and 2,6,10,15,19,23-hexamethyl-, (all-E) were known to posesss antimicrobial activity. Several other compounds with a number of therapeutic properties were also reported in the present study.