Abstract
Emergence of resistant microbes to conventional antibiotics and increased emphasis on health-care costs has raised the concern for the development of new effective antimicrobial reagents. Silver nanoparticles being an excellent broad-spectrum antibacterial agent could be considered as a suitable alternative for existing antibiotic. This study demonstrates the extra-cellular synthesis of stable silver nanoparticles using supernatant of Lysinibacillus varians. The synthesized silver nanoparticles were characterized by using UV–visible spectrum analysis, X-ray diffraction, Transmission electron microscopy (TEM) and FT-IR analysis. The synthesized silver nanoparticles showed a peak around 420 nm. TEM analysis revealed that the size of silver nanoparticles was in the range of 10–20 nm. Silver nanoparticles carry a charge of −39.86 mV, which confirmed the stability of silver nanoparticles. The biologically synthesized silver nanoparticles showed antimicrobial activity against Gram-positive, Gram-negative bacteria and fungi. Therefore, the current study reveals an efficient and eco-friendly synthesis of silver nanoparticles by L. varians with potent antimicrobial activity.
Highlights
Nanotechnology is an interdisciplinary field which impacts significantly all aspects of human’s life (Mohanpuria et al 2008; Liu et al 2011)
This paper reports the extracellular biosynthesis of silver nanoparticles with their potent antimicrobial activity
The soil sample from metal contaminated site was used for the isolation of bacteria capable to synthesize silver nanoparticles
Summary
Nanotechnology is an interdisciplinary field which impacts significantly all aspects of human’s life (Mohanpuria et al 2008; Liu et al 2011). This field deals with the synthesis and applications of nanomaterials in various areas (Duran et al 2005). Microbial synthesis of metal nanoparticles can take place either intracellularly or extracellularly (Ahmad et al 2007; Jain et al 2011; Kalishwaralal et al 2010; Pugazhenthiran et al 2009; Saifuddin et al 2009). At the same time extracellular biosynthesis is cheap and requires simpler downstream processing. This paper reports the extracellular biosynthesis of silver nanoparticles with their potent antimicrobial activity
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