Eco-friendly synthesis of silver nanoparticles using seeds of Sorghum bicolor

Abstract

The present study was aimed to synthesize nanoparticles using a facile environmentally benevolent green synthetic route. They were synthesized by reducing the silver ions present in the solution of silver nitrate (0.001M) with aqueous seed extract of Sorghum bicolor. The aqueous extract was subjected for preliminary phytochemical analysis according to standard methods. Characterization of silver nanoparticles was done by using UV, FTIR, SEM and TEM analysis. Further synthesized silver nano particles were screened for antibacterial activity at 50, 100, 150, 200^g/ml concentrations against two Gram positive bacteria (Staphylococcus aureus, Bacillus subtilis) and one Gram negative bacteria (Escherichia coli). The synthesized nanoparticles had shown highest antibacterial activity against Staphylococcus aureus and moderate anti-bacterial activity against Bacillus subtilis and Escherichia coli. Thus this study can be applied for rapid, cost effective and eco-friendly green synthesis of silver nanoparticles using seeds of Sorghum bicolor.