Abstract
Some endophytic fungi live in plant tissue and does not caused any damage on their host plant. The endophytic fungi could produce antimicrobial secondary metabolites. Some endophytic fungi have been isolated from a medicinal plant Physalis angulata, i.e: Penicillium verrucosum, Colletotrichum alienum, Fusarium subglutinans, Aspergillus nidulans, Mycelia sterilia 1, Mycelia sterilia 2, and Rhizoctonia sp. The purpose of this research are: 1) to examine the antimicrobial activity of each endophytic fungi species toward Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and, 2) to determine the endophytic fungi species that have highest antimicrobial activity. Each endophytic fungi isolates were inoculated in Potato Dextrose Broth (PDB) medium and shaked in 120 rpm, during 7 x 24 hours, then the broth culture centrifugated in the rate of 3000 rpm for 10 minutes. The supernatants from each endophytic fungi were treat to the bacteria to know the antimicrobial activity by agar diffusion method. Afterwards the antibaterial effect of each endophytic fungi species were measured. Then the highest antimicrobial effect were determined. The research results shows that: 1) each endophytic fungi species have antimicrobial activity towards B. subtilis, E. coli, S. aureus, and; 2) Penicillium verucossum secondary metabolites have the highest antimicrobial activity.
Highlights
IntroductionPeople try to find an alternative material from nature for antibiotic substance
Some research about antimicrobial activity of medicinal plants have been done
It was proved that the secondary metabolites in liquid culture of each endophytic fungi species isolated from H. acuminatum have antibacterial effect towards Staphylococcus aureus, and Bacillus subtilis [6]
Summary
People try to find an alternative material from nature for antibiotic substance. Altough the endophytic fungi live in the host plants it does not cause any damage, but the fungi could produce some antimicrobial secondary metabolites. The last research results shows that some endophytic fungi species live in Hedychium acuminatum tissue, it was: Fusarium oxysporum, Rhizoctonia sp., Fusarium solani, Fusarium semitectum, Colletotrichum alienum, Colletotrichum ti, Colletotrichum aotearoa, Colletotrichum gloesporoides, and Aspergillus parasiticus. These fungi species have an ability to produce antimicrobial secondary metabolites as well as the host plant produce, i.e: alkaloid, flavonoid, terpenoid, and tannin [5]. It was proved that the secondary metabolites in liquid culture of each endophytic fungi species isolated from H. acuminatum have antibacterial effect towards Staphylococcus aureus, and Bacillus subtilis [6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
