Abstract
Objective: The aims of the present study were to screen the actinobacteria with high potential ability to produce secondary metabolites that have inhibitory activity against plant pathogenic fungi, Magnaporthe oryzae. Production of secondary metabolites was analysis by thin-layer chromatography and bioautography assay.
 Methods: Screening and selection of potential Streptomyces sp. morphological, cultural, physiological, and biochemical characterization of the screened isolate was carried out. Antifungal compound was confirmed by bioautography assay.
 Results: Bioautography method use in this study was found to be antifungal fraction from the crude extract. Antifungal secondary metabolites can be readily located on the plates by observing clear zones where active compounds inhibit fungal growth.
 Conclusion: The bioautography assay shows that this isolates can produce antifungal compound. Therefore, this isolate proves to be a promising microbe which can be further studied for its applications a biocontrol agent against rice blast fungi.
Highlights
Rice is a staple food source for more than four billion people worldwide
Rice is vulnerable to diseases, the most destructive among them being rice blast disease, which is caused by the fungus Magnaporthe oryzae
Screening of potential actinobacterial strain Primary screening Among the 20 actinobacterial isolates that were subjected to antagonistic test by primary screening method, six isolates showed potential inhibition against M. oryzae fungal pathogen
Summary
Rice is a staple food source for more than four billion people worldwide. rice is vulnerable to diseases, the most destructive among them being rice blast disease, which is caused by the fungus Magnaporthe oryzae (anamorph Pyricularia oryzae). The antibiotic Oligomycin A was first isolated from Streptomyces diastatochromogenes and was controlling several other plant pathogenic fungi such as Botrytis cinerea, Cladosporium cucumerinum, Colletotrichum lagenarium, Phytophthora capsici, Alternaria alternata, and Aspergillus niger in addition to M. oryzae [1,2,3]. Other compounds such as Pyrroles (Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-) are commonly found in various actinobacterial species [4,5]. Pyrrolo[1,2-a] pyrazine-1,4dione,hexahydro-3-(phenylmethyl) was found to be able to protect plants from disease causing plant pathogenic fungi and bacteria [6]
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