Abstract
The treatment of diabetes mellitus using drug medications have side effects such as weight gain, hypoglycemia, the risk of cardiovascular or all-cause mortality. These limitations have become one reason to use complementary therapy from plant extract, such as Vernonia amygdalina as an antidiabetes. This research aimed to investigate the effect of V. amygdalina leaf extracts as antidiabetic and antioxidant agents by measuring the fasting Blood Glucose Levels (BGLs), IC50 value and the profiles of phytochemical constituents using LC-MS analysis. We used 24 Sprague-Dawley rats (200-300 g) divided into six groups: normal group, positive control group and negative control group. The other three groups were leaf extract groups in different doses: 200, 400 and 600 mg/kg BW dose of leaf extract groups, respectively. The antidiabetic effects were tested based on BGLs were observed for 16 days and examined on day 4, 8, 12 and 16. The rat renal histological changes were observed under an electron microscope using Hematoxylin Eosin (HE) dye. V. amygdalina leaf extract showed an effect in reducing BGL on diabetic rats with an effective dose of 200 mg/kg. Renal histopathology result showed necrosis on proximal tubules in all treatment except the normal group. In conclusion, the administration of V. amygdalina leaf extract can reduce BGL. However, it did not show any improvement in the histopathological structure of the kidney.
Highlights
Diabetes is a chronic disease caused by the pancreas inability to produce enough insulin hormones or ineffective use of insulin hormones in the body
Based on the data from research of basic health (Riskesdas), the proportion of people diagnosed with Diabetes Mellitus (DM) was 6.9% of the total population, which is approximately 12,191,564 people with age >15 years old were diagnosed DM in Indonesia in the year 2013
Data from International Diabetes Federation (IDF) showed that people with DM kept on rising and Indonesia was in the 7th place for the country with the most people with DM from 2013 to 2015 (Indonesia, 2015; Atlas, 2015)
Summary
Diabetes is a chronic disease caused by the pancreas inability to produce enough insulin hormones or ineffective use of insulin hormones in the body. Based on the data from research of basic health (Riskesdas), the proportion of people diagnosed with Diabetes Mellitus (DM) was 6.9% of the total population, which is approximately 12,191,564 people with age >15 years old were diagnosed DM in Indonesia in the year 2013. Data from International Diabetes Federation (IDF) showed that people with DM kept on rising and Indonesia was in the 7th place for the country with the most people with DM from 2013 to 2015 (Indonesia, 2015; Atlas, 2015). Endocrinology Society (Perkeni) reported that DM main problem in Indonesia is not-routinely taking antidiabetes drugs or insulin injection. Problem-related to DM is the lack of access to the health centers, public health center facilities and infrastructure for people with DM in Indonesia (Soewondo et al, 2013). Nephropathy is one of DM complications in the kidney (Chawla et al, 2016; Targher et al, 2018)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
