Abstract
In this study we investigated effects of natural extract from the black tea Camellia sinensis (BTE) against human colon carcinoma cell line HT-29, human breast carcinoma cell line MCF-7, human alveolar carcinoma cell line A549 and healthy cell line NIH-3T3. We identified concentration range for cytotoxic/antiproliferative effects using MTT assay and the trypan blue assay, gel electrophoresis we employed to determine the type of cell death induced by BTE and DNA damage we determined by comet assay. Different concentrations of the extract (0.00078 - 5 μg/mL) we added to the cultured cells and incubated for 216 h. BTE showed cytotoxic effects against all carcinoma cell lines, however HT-29 and MCF-7 cells were more sensitive than A549. BTE showed no antiproliferative effect against healthy cells NIH-3T3 at tested concentrations. We found no apoptotic cell death in HT-29 and MCF-7 cells after 72 h of incubation in case of single administration of BTE but in case of repetitive administration of BTE (BTE was added to the cells each day) we found apoptotic cell death in HT-29 after 72 h incubation. BTE induced also DNA strand breaks and oxidative damage to DNA in carcinoma cells HT-29 and MCF-7.
Highlights
Black tea has a long history of use dating back to China approximately 5,000 years ago
Similar pattern with higher Half maximal inhibitory concentration (IC50) concentrations can be observed in MCF-7 cells
We observed no antiproliferative effects of black tea extract (BTE) on mouse healthy fibroblast cell line NIH-3T3 during 72 h of influence at BTE concentrations 0.00078 – 5 μg/mL
Summary
Black tea has a long history of use dating back to China approximately 5,000 years ago. It is made from the dried leaves of Camellia sinensis, a perennial evergreen shrub formerly known as Thea sinensis. Black tea results from the oxidation of Camellia sinensis leaves. Black tea is used for treating headaches, low blood pressure, preventing heart disease, including atherosclerosis and heart attack, preventing Parkinson’s disease, reducing the risk of stomach and colon cancer, lung, ovarian and breast cancers (Lee and Foo 2013). The aim of our study was to evaluate the potential anticancerogenic effect of the black tea extract (BTE) on different types of carcinoma cell lines
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