Abstract
Objective To study the role of membrane cytoskeleton linker Ezrin in the metastasis of human breast carcinoma cell line MCF-7 facilitated by CC chemokine-5 (CCL5). Methods Two pairs of small interfering RNA (siRNA) targeting Ezrin were transfected into the human breast carcinoma cell line MCF-7cells. Western blotting was used to detect the down-regulation of Ezrin mRNA and protein. The invasion assays were performed on breast cancer cells in the presence of CCL5. Western blotting was used to examine total Ezrin and phosphorylated Ezrin expression in the MCF-7 cells responded to CCL5 at different time points. Results The cells responded chemotactically to CCL5 in a dose-dependent manner (F=31. 62,P 0.05). Conclusion Chemotaxis and invasion of breast cancer cells can be facilitated by CCL5 expression. Our results show that a potential role for Ezrin in the process of the breast cancer cells migration, invasion and metastasis responds to the chemokine CCL5. Key words: Breast neoplasm; Invasion; RNA interference
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