Antibiotic susceptibility and biofilm formation in multidrug resistant Pseudomonas aeruginosa

Abstract

one of the Gram-Negative pathogens, serves a crucial organism in nosocomial outbreaks. The properties of this bacteria including adaptability, biofilm formation, etc. make this organism develop resistance against various antibiotic drugs. The current study aims to screen the multidrug resistant isolates for biofilm formation and to study the relationship of drug resistance and development of biofilm. Clinical samples growing in culture were screened for antibiotic susceptibility pattern using disc diffusion method. The multidrug resistant isolates of identified using culture and standard microbiological tests were included in the study and were tested for biofilm formation using micro titre plate assay: Out of the 224 clinical samples growing in culture, 100 isolates were found to be multidrug resistant (MDR). 42% of the isolates showed resistance to Cefepime followed by 26% isolates were resistant to Levofloxacin. 88% of the MDR isolates produced biofilm and among these 75% produced strong biofilm, 10% of the isolates produced weak biofilm and 3% of the isolates produced moderate biofilms. The present study observed that majority of the MDR isolates were found to be biofilm producers. Thus, biofilm production is said to be one of the important properties of the organism which could be attributed to their multi drug resistance.