Abstract
Pseudomonas aeruginosa induced infections are becoming very common and bacterial resistance to antibiotics causes a significant challenge to health systems globally by imposing massive financial burdens. Finding antibiotics substitutes with better antibacterial activity and lower risk are highly desired. Metallic nanoparticles have been found to be very attractive antibacterial agents. In current research, Carbon-wrapped Copper-Nickel nanoparticles were prepared and characterized using Fourier Transform Infrared spectroscopy (FT-IR) and X-ray diffraction analysis. Propylene glycol, sodium alginate, and 2-phenoxy ethanol-based hydrogels were loaded with two different concentrations of Copper-Nickel nanoparticles 30 mg/ml (ALG-30) and 50 mg/ml (ALG-50). The synthesized gels were found to be cytocompatible in cell culture study using fibroblasts. Carbon-wrapped Copper-Nickel nanoparticles, ALG-30 and ALG-50 showed remarkable antibacterial activity against both Gram-positive (S. aureus and MRSA) and Gram-negative (E. coli and P. aeruginosa) bacteria. Pseudomonas aeruginosa and MRSA biofilms were significantly decreased when exposed to nanoparticles and hydrogels (ALG-30 and ALG-50) for 24 hours. In full thickness infected skin wound model in rat, Pseudomonas aeruginosa infected wound was almost 97 % healed with ALG-30 over 16 days. In ALG-30 treated group, H & E stained tissues depicted enhanced angiogenesis, reduced inflammation and stimulate re-epithelialization, while Masson trichrome stained tissues showed increased collagen deposition. Overall, Carbon-wrapped Copper-Nickel nanoparticles embedded hydrogels exhibited excellent antimicrobial activity and holds a considerable potential to alleviate the current antibiotic resistance crisis in chronically infected wounds and expected widespread use in clinical practice.
Published Version
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