Abstract
Background: Escherichia coli, sometimes known as E. coli, is a gram-negative bacterium that is typically present in people's intestinal flora. Some forms of E. coli can cause sickness, while the majority of these types are benign and aid in digestion. By creating biofilms, bacteria are able to defend themselves against mechanical, chemical, and biological assaults, giving them exceptional survival benefits. The scented medicinal grass known as lemon grass belongs to the Cymbopogon genus. Lemongrass essential oils include bioactive phytoconstituents that have a wide range of therapeutic benefits, including antibacterial, anticancer, antioxidant, insecticidal, and antimalarial actions. Methods: The aim of this study is to evaluating the antibacterial activity of (C. citrates) essential oil leaves extract and assessment of its effect on fimA and papC genes in E. coli. Gas chromatography and mass spectrometry analyses of the C. citrates essential oil extract. Forty-eight isolates of E. coli from different sources such as (urine, blood and stool. the diagnosis was confirmed using VITEK-2. The experiments use in this study is (Antibiotic susceptibility test, Disc diffusion method, Total phenolic content, Determination of Minimum Inhibitory Concentration (MIC) of C. citrates L. m biofilm formation and Genetic analysis). Results: The objective of this study is to assess the antibacterial and antibiofilm properties of extracts derived from the leaves of C. citrates and the effect on gene expression of fimA and papC genes after treatment with sub-MIC of C. citrates leaves extracts. Conclusion: The finding of this study showed that (90%) of the isolates had FimA while PapC was present in (60%) of the total isolates. The gene expression revealed that there was a decrease in the expression of Fim A and PapC genes.
Published Version
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