Abstract
Chuanxiong Rhizoma and Cyperi Rhizoma (CRCR), an ancient and classic formula comprised of Chuanxiong Rhizoma and Cyperi Rhizoma in a weight ratio of 1:2, has long been used for curing migraine. This study aimed to explore their anti-migraine effect and active constituents. A nitroglycerin (NTG)-induced migraine model in rats was established to evaluate pharmacological effects. Cerebral blood flow was detected by a laser Doppler perfusion monitor. The levels of endothelin-1 (ET-1), γ-aminobutyric acid (GABA), nitric oxide synthase (NOS), nitric oxide (NO), 5-hydroxytryptamine (5-HT), 5-hydoxyindoleacetic acid (5-HIAA), calcitonin gene-related peptide (CGRP) and β-endorphin (β-EP) were quantified with enzyme-linked immunosorbent assay. CGRP and c-Fos mRNA expression were quantified with quantitative real-time polymerase chain reaction. A UPLC-MS/MS method was developed and validated for the simultaneous quantification of active constituents in rat serum and cerebral cortex. CRCR significantly increased cerebral blood flow, decreased the levels of ET-1, GABA and NOS, and increased the levels of 5-HT, 5-HIAA and β-EP in NTG-induced migraine rats. CGRP levels and CGRP mRNA expression, as well as c-Fos mRNA expression in the brainstem were markedly down-regulated with the treatment of CRCR. After oral administration of CRCR, ferulic acid (FA), senkyunolide A (SA), 3-n-butylphthalide (NBP), Z-ligustilide (LIG), Z-3-butylidenephthalide (BDPH), cyperotundone (CYT), nookatone (NKT) and α-cyperone (CYP) were qualified in rat serum and cerebral cortex. The above results suggested that CRCR showed powerfully therapeutic effects on migraine via increasing the cerebral blood flow, decreasing the expression of CGRP and c-Fos mRNA, and regulating the releasing of ET-1, GABA, NOS, 5-HT, 5-HIAA, CGRP and β-EP in the serum and brainstem, consequently relieving neurogenic inflammation. The active constituents in CRCR for treating migraine were FA, SA, NBP, LIG, BDPH, CYT, NKT and CYP. These findings contributed for the further use of CRCR as a combinational and complementary phytomedicine for migraine treatment.
Highlights
Migraine is a chronic paroxysmal neurological disorder characterized by recurrent attacks of predominantly unilateral throbbing head pain [1]
This study indicated that Chuanxiong Rhizoma and Cyperi Rhizoma (CRCR) exerted powerfully preventive and therapeutic effects on migraine via increasing the cerebral blood flow, decreasing the expression of calcitonin gene-related peptide (CGRP) and c-Fos mRNA, and regulating the releasing of ET-1, GABA, nitric oxide synthase (NOS), 5-HT, 5-hydoxyindoleacetic acid (5-HIAA), CGRP and β-EP in the serum and brainstem, relieving neurogenic inflammation
The present study indicated that CRCR showed significantly therapeutic effects on migraine via increasing cerebral blood flow, decreasing the expression of CGRP and c-Fos mRNA, and regulating the releasing of ET-1, GABA, NOS, 5-HT, 5-HIAA, CGRP and β-EP in the serum and brainstem
Summary
Migraine is a chronic paroxysmal neurological disorder characterized by recurrent attacks of predominantly unilateral throbbing head pain [1]. There are different types of medicines in the treatment of acute migraine attacks, such as triptans, ergotamines and analgesics [2,3]. These agents often have an insufficient efficacy and are accompanied with a numerous side effects [4,5,6]. The effective management of migraine remains a moving field and needs to be sharply into focus. There is a growing body of evidence supporting the efficacy of complementary and alternative medicine approaches in the management of migraine. Many herbs and herbal formulas are effective for migraine prevention and treatment [7]
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