Abstract
Citrus sinensis, Persea americana and Allium sativum are good sources of large number of various bioactive substances including antioxidants. Antioxidants play an essential role in the prevention and treatment of type 2 diabetes and other metabolic diseases. This study was conducted to evaluate and compare the anti- lipid peroxidation, anti-hemolytic and antioxidant capacity of aqueous extracts of the three plants in vitro. Polyphenolic and flavonoids content of aqueous extracts (EA) of plants were determined. The anti-lipid peroxidation was evaluated on homogenates of rat liver. Anti-haemolytic effects were assessed using erythrocyte system model induced free radicals from human red blood cells. Total antioxidant capacity and antiradical or scavenging capacity were evaluated on biological free radical systems (OH, NO.) and synthetic radicals (DPPH and ABTS+). Results showed that, different extracts possess variable amounts of polyphenolic compounds. They exhibited significant anti-hemolytic and anti-lipid peroxidation activities. Aqueous extracts of Citrus sinensis are powerful scavenger of OH. (IC50=1.05x10-3mg/ml) and NO. (IC50= 1.29x10-3 mg/ml) free radicals while Persea americana seed extract possesses the highest anti-lipid peroxidation capacity (59.72%) and the best scavenging capacity against DPPH and ABTS. Red blood cells were highly protected (at 97.87%, p<0.05) by Allium sativum extract at 1mg/mL and Citrus sinensis (87.7%, p<0.05). Therefore, anti-hemolytic, anti-lipid peroxidation effects of the aqueous extracts of the three plants can justify their use and efficacy in alternative treatment of metabolic diseases.
Highlights
Type II diabetes mellitus (T2DM) is a metabolic disorder characterized by prolonged hyperglycemia, insulin resistance and/or reduced insulin sensitivity (Kooti, Maryam, Zahra, Damoon & Majid, 2016)
This study aims at comparing the anti-hemolytic effect of the three plants based on radical-induced erythrocyte lyses; to assess the anti-lipid peroxidation effects; to determine the antioxidant and scavenging capacity against free radicals in vitro
They were followed by Citrus sinensis and the lowest percentage of inhibition was observed with Allium sativum (Figure 3)
Summary
Type II diabetes mellitus (T2DM) is a metabolic disorder characterized by prolonged hyperglycemia, insulin resistance and/or reduced insulin sensitivity (Kooti, Maryam, Zahra, Damoon & Majid, 2016). In T2DM patients, hemorheological parameters are often disturbed due to excess glucose concentration in the blood. This affects haematocrit, plasma proteins, erythrocyte aggregation and deformability (Youn et al, 2017). All these disturbances affect blood viscosity of diabetic patients compared to healthy patients (Young, Michael, Mooney & Daniel, 2008). Erythrocytes or red blood cells (RBC) play a key role in the metabolism as well as in oxygen (O2) and drug transportation. Attacks of polyunsaturated fatty acid of lipids and proteins found in erythrocyte membrane are responsible for hemolysis and can justify the greater osmotic fragility observed in diabetic patients. Cell membranes of RBC are made up of http://jfr.ccsenet.org
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