Abstract
Objective: To investigate the population of endophytic fungi accompanying to Nerium olender plant. Materials and Methods: The study was conducted on three different sites in two governorates for three consecutive months with five samples from each site. The identification of the fungi was carried out using microscopic and molecular methods while the isolation, identification of fungal pathogen and preparation of aqueous extract were also successfully done. Results: 14 species of fungi (Penicillium notatum, Rhizopus nigricans, Rhizopus stolonifera, Fusarium Solani, Fusarium oxysporum, Alternaria sp., Cladosporium sp .and Curvularia sp.), while the other sex species were identified by DNA investigation method and where (Aspergillus spp. (40%), Penicillium spp. (20%), Rhizopus spp. (12%), Fusarium spp. (10%), Alternaria sp. (4%), Mucor circinelloides (4%), Neurospora Crassa (3%), Cladosporium sp. (1%) and Curvularia sp. (1%). Conclusions: The highest percentage of inhibition was for fungi Aspergillus Flavus, Penicillium commune and Mucor circinelloides which amounted to 100% and from the third day of growth until the completion of the control dish, then Aspergillus niger and Rhizopus stolonifera, with a 90% on the seventh day of growth, and Penicillium commune, Rhizopus nigricans with a rate ranging between 82-68%, while the rest of the fungi showed an antagonistic effect, but with rates less than 50%.
Published Version
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