Animal Cell Electroporation and Electrofusion Protocols

Abstract

Part I. Theory and Instrumentation. Electroporation Theory: Concepts and Mechanisms. Effects of Pulse Length and Strength on Electroporation Efficiency. Instrumentation. Part II. Electroporation Protocols. The Introduction of Proteins into Mammalian Cells by Electroporation. Electroporation of Antigen-Presenting Cells for T-Cell Recognition and Cytotoxic T-Lymphocyte Priming. Electroporation of Antibodies into Mammalian Cells. Electroporation of Adherent Cells In Situ for the Introduction of Nonpermeant Molecules. Electrotransformation of Chinese Hamster Ovary Cells. Electroporation of Rat Pituitary Cells. Electroporation of Plasmid DNA into Normal Human Fibroblasts. Electroporation-Mediated Gene Transfer into Hepatocytes. Electroporation of Human Lymphoblastoid Cells. The Use of Electroporated Bovine Spermatozoa to Transfer Foreign DNA into Oocytes. Electroporation of Embryonic Stem Cells for Generating Transgenic Mice and Studying In Vitro Differentiation. Electrotransfection with 'Intracellular' Buffer. Effect of Cis-Located Human Satellite DNA on Electroporation Efficiency. Quantitation of Transient Gene Expression. Stable Integration of Vectors at High Copy Number for High-Level Expression in Animal Cells. Electroporation of Drosophila Embryos. Transformation of Fish Cells and Embryos. Electroporation of Cardiac Cells. Electroporation for Gene Therapy. Part III. Electrofusion Protocols. Electrofusion of Mammalian Cells. Stabilizing Antibody Secretion of Human Epstein Barr Virus-Activated B-Lymphocytes with Hybridoma Formation by Electrofusion. Electrofusion of Mammalian Oocytes and Embryonic Cells. Nuclear Transfer in Bovine Embryos. Electrofusion of Mouse Embryos to Produce Tetraploids. Spectrofluorometric Assay for Cell-Tissue Electrofusion. Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products. Index.