Abstract

Part I. Theory And Instrumentation. Electroporation Theory: Concepts and Mechanisms. Effects of Pulse Length and Strength on Electroporation Efficiency. Instrumentation. Part II. Electroporation Protocols. Electroporation of Agrobacterium tumefaciens. Electroporation of DNA into the Unicellular Green Alga Chlamydomonas reinhardtii. Pollen Electrotransformation in Tobacco. Electroporation of Tobacco Leaf Protoplasts Using Plasmid DNA or Total Genomic DNA. Electroporation of Brassica. Transformation of Maize by Electroporation of Embryos. Transient Gene Expression Analysis in Electroporated Maize Protoplasts. Reporter Genes and Transient Assays for Plants. Part III. Electrofusion Protocols. Electrofusion of Plant Protoplasts: Selection and Screening for Somatic Hybrids of Nicotiana. Protoplast Electrofusion and Regeneration in Potato. Polymer- Supported Electrofusion of Protoplasts: A Novel Method and a Synergistic Effect. Index.

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