Abstract
Cell electro-fusion includes four steps, cell alignment, cell electroporation, reconstruction of cytomembrane and cytoplasm exchange. The cell alignment and electroporation steps are highly related to the intensity and distribution of the electric field, which depend on the applied voltage as well as the microelectrode structure. The microelectrode structures were first evaluated based on the numerical analysis of the electric field and the transmembrane potential induced on biological cells when the cell electroporation and electro-fusion were performed based on different designs of microelectrodes. Microelectrodes in a micro-cavity geometry can induce electroporation around the contact area of the paired cells for high-yield electro-fusion. Microfluidic chips with co-planar microelectrodes and microelectrodes within micro-cavities have been fabricated and tested for electro-fusion of Myoblast cells, and the experimental results confirmed the numerical analysis.
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