Aneuploidy in rabbit males: semen traits and fertility

Abstract

Chromosomal analyses were performed from peripheral blood samples from 20 adult rabbit males from Line R. This line has been selected for growth rate after weaning through 25 generations. Seminal characteristics and reproductive outcomes from these males were also examined. The chromosomal analysis results showed that one male had an aneuploidy rate of 16% (male A). The aneuploidies found represented both hypo- and hyper- haploidy. Differences between the male A and the contemporary males (males N), in fertility at 12 days post-insemination (44% vs. 66%) and at birth (31% vs. 59%) were observed. The male A also showed a high percentage of pregnancy losses (29% vs. 12%). For seminal characteristics, the percentage of abnormal spermatozoa was statistically different (P<0.05) between male A and males N (34+/-4 vs. 18+/-2), and concentration differed significantly between males, showing the male A reduced fewer spermatozoa than males N (67+/-23 vs. 172+/-10, x106/ml). Motility and kinematic parameters revealed no differences between males. Differences between male reproductive performance found in this study could be explained by the effect of aneuploidy on spermatogenesis and its deleterious effect on male reproduction.