Abstract
Sweeteners have been used in food for centuries to increase both taste and appearance. However, the consumption of sweeteners, mainly sugars, has an adverse effect on human health when consumed in excessive doses for a certain period, including alteration in gut microbiota, obesity, and diabetes. Therefore, the application of non-nutritive sweeteners in foodstuffs has risen dramatically in the last decade to substitute sugars. These sweeteners are commonly recognized as high-intensity sweeteners because, in a lower amount, they could achieve the same sweetness of sugar. Regulatory authorities and supervisory agencies around the globe have established the maximum amount of these high-intensity sweeteners used in food products. While the regulation is getting tighter on the market to ensure food safety, reliable analytical methods are required to assist the surveillance in monitoring the use of high-intensity sweeteners. Hence, it is also necessary to comprehend the most appropriate method for rapid and effective analyses applied for quality control in food industries, surveillance and monitoring on the market, etc. Apart from various analytical methods discussed here, extraction techniques, as an essential step of sample preparation, are also highlighted. The proper procedure, efficiency, and the use of solvents are discussed in this review to assist in selecting a suitable extraction method for a food matrix. Single- and multianalyte analyses of sweeteners are also described, employing various regular techniques, such as HPLC, and advanced techniques. Furthermore, to support on-site surveillance of sweeteners’ usage in food products on the market, non-destructive analytical methods that provide practical, fast, and relatively low-cost analysis are widely implemented.
Highlights
The results show that consumption over ten years is associated with the incidence of type 2 diabetes
There is a safe limit for non-nutritive sweeteners in water that does not have adverse effects on aquatic organisms, for example, sucralose, which shall not be more than 1000 mg L−1 [12,38,39]
Some rapid determination methods were developed on the basis of advanced analytical techniques, such as electrophoresis (CE), chromatography (UHPLC), vibrational spectroscopy (FT-NIR), and sensor and biosensor combined with chemometrics (Table 4)
Summary
Sweeteners are originally derived from natural resources such as fruits and vegetables, while currently, a number of artificial sweeteners are available by chemical synthesis. The application of non-nutritive sweeteners in foodstuffs has risen dramatically in the last decade to substitute sugars These typical sweeteners are commonly recognized as high-intensity sweeteners because, in a lower amount, they could achieve the same sweetness of sugar. Because of this reason, non-nutritive sweeteners have been widely used in industries to prepare various foods claimed as “diet” or “light” products. Non-nutritive sweeteners have been widely used in industries to prepare various foods claimed as “diet” or “light” products These relatively low-calorie products are attractive to consumers who are maintaining body weight or controlling blood sugar in the management of diabetes. Note: Joint FAO/WHO Expert Committee on Food Additives (JECFA), Codex Alimentarius Commission (CAC), Food and Drug Administration (FDA), Food Standards Australia New Zealand (FSANZ), and National Agency of Drug and Food Control (NADFC)
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