Analysis of the volatile constituents of flos chrysanthemi indici in Yangling demonstration zone of Shaanxi by SPME/GC/MS

Abstract

Objective To analyze the chemical constituents of the volatile constituents of Flos chrysanthemi indici. Methods The volatile constituents in Flos chrysanthemi indici was extracted by the method of Solid phase microextraction (SPME). The components of the volatile constituents were separated and identified by gas chromatography-mass spectrometry (GC-MS). The relative content of each component was determined by area normalization. Results Fifty different compounds were isolated and identified, accounting for 97.17% of the total volatile constituents. The main components in volatile constituents of Flos chrysanthemi indici were eucalyptol (24.72%), a-Myrcene (15.78%), 1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene-, (Z)- (10.44%), Camphor (10.05%), Naphthalene, 1,2,3,4,4a,5,6,8a-octahydro-7-methyl-4-methylene-1-(1-methylethyl)-, (1a,4aa,8aa)- (4.81%), a-Phellandrene (4.43%), 1R-a-Pinene (4.28%), Bornyl acetate (2.56%), Bicyclo [3.1.0] hexane, 4-methylene-1-(1-methylethyl)- (1.59%), 1H-Benzocycloheptene, 2,4a,5,6,7,8-hexahydro-3,5,5,9-tetramethyl-, (R)-(1.47%). Conclusions The SPME technology can be used for volatile component analysis of wild chysanthemum,and it can help to improve the sensitivity and accuracy. Key words: Chrysanthemi indici flos; Volatile oil determination (TCD); Solid phase microextraction; Gas chromatography-mass spectrometry