Analysis of Secondary Metabolites Released by Pseudomonas fluorescens Using GC-MS Technique and Determination of Its Anti-Fungal Activity

Abstract

Pseudomonas fluorescens is a common gram-negative, rod-shaped bacterium [1]. It belongs to the Pseudomonas genus. The objectives of this study were analysis of the secondary metabolite products and evaluation antimicrobial activity. Twenty four bioactive compounds were identified in the methanolic extract of Pseudomonas fluorescens. GC-MS analysis of Pseudomonas fluorescens revealed the existence of the cis-5, 8, 11, 14, 17-Eicosapentaenoic acid, 12, 15-Octadecadiynoic acid, methyl ester, 7-epi-cis-sesquisabinene hydrate, α-D-Glucopyranoside, O-α-D-glucopyranosyl, Acetamide, N-methyl-N-[4-[2-acetoxymethyl-1-pyrrol, Acetamide, N-methyl-N-[4-[2-fluoromethyl-1-pyrrolid, Phen-1, 4-diol, 2, 3-dimethyl-5-trifluoromethyl, Geranyl isovalerate, Quinazoline, 4-methyl, Pentetic acid, trans-13-Octadecenoic acid, 9-Hexadecenoic acid, 13-Hexyloxacyclotridecan-2-one, 7-Methyl-Z-tetradecen-1-ol acetate, cis-13-Eicosenoic acid, Didemin B, Hexadecanoic acid, 1-(hydroxymethyl)-1, 2-ethaned, and Ethyl iso-allocholate. Cassia angustifolia (Crude) was very highly active (6.007±0.20) mm against Pseudomonas fluorescens. The results of anti-fungal activity produced by Pseudomonas fluorescens showed that the volatile compounds were highly effective to suppress the growth of Trichoderma horzianum (5.019±0.18). Pseudomonas fluorescens produce many important secondary metabolites with high biological activities.