Abstract
Transition metal complexes have been used in medicine for several decades, but their intracellular effects are not yet fully elucidated. Therefore, in this study, we investigate biomolecular changes induced by a palladium(II) complex in cervical carcinoma (HeLa) cells as a model to study the subtle changes caused by transition metal ions ingested by the cells. The impact of dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl2], was studied by synchrotron radiation-based Fourier transform infrared (SR FTIR) spectroscopy, a powerful tool for studying alterations in cellular components’ biochemical composition and biomolecular secondary structure on a single-cell level. A spectral analysis, complemented by statistics, revealed that the Pd(II) complex considerably affected all major types of macromolecules in HeLa cells and induced structural changes in proteins through an increased formation of cross-β-sheets and causes structural rearrangement in deoxyribonucleic acid (DNA) through potential chromosome fragmentation. Although a certain level of lipid peroxidation was detectable by SR FTIR spectroscopy and confirmed by an analysis of cellular lipids by matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry, the oxidative stress is not a significant mechanism by which Pd(II) expresses the effect on the HeLa cells.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call