Abstract
Coprinus atramentarius Fries was grown in surface culture on selected media. The quantity of mycelium formed was small; no carpophores were produced. Addition of a low concentration of disulfiram to cultures of the fungus had no apparent effect on the development of the organism, and the added disulfiram disappeared from the cultures within sixty days. Concentrated extracts of the media and mycelia of the cultures of the fungus, as well as extracts of naturally-occurring carpophores, were investigated for the presence of disulfiram by means of a paper partition chromatographic method. It is concluded that the disulfiram-Iike physiological activity which was exhibited by the naturally-occurring carpophores of C. atramentarius is due to some agent other than disulfiram.
Published Version
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