Abstract
SummaryThe effects of different concentrations of adenine and kinetin on the development of carnation meristem tips, cultured on artificial substrate, are reported. 0.1 ppm kinetin added to a liquid substrate induced a marked inhibition of root formation and 1 ppm kinetin inhibited it completely. In both instances, kinetin promoted lateral bud development. Lower concentrations had no apparent effect on organogenesis. Only 10 ppm adenine inhibited root formation, lower concentrations had no apparent effect; none of these concentrations induced lateral bud development. The inhibition of root formation induced by 0.1 ppm kinetin was significantly reduced by 1.0 ppm adenine, but lateral bud formation was not affected. It was concluded that there was a threshold concentration of kinetin below which the effects on organogenesis disappeared, and that lateral bud formation was independent of root development.
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