Abstract
The physiological and pathological processes in lungs indicated that ONOO- acted as an informative indicator for pulmonary functioning status with the corresponding dynamics, especially in permissive hypercapnia. Herein, for the peroxynitrite (ONOO-) detection and imaging in living lung cells, an imidazo-pyridin derived fluorescent probe IDPD-ONOO was developed. IDPD-ONOO recognized ONOO- to exhibit a corresponding fluorescence response at 490nm with the excitation wavelength at 355nm. The advantages of the developed probe included practical linear range (0-100μM), high sensitivity (Limit of Detection 0.005μM), rapid response (within 15min), pH tolerance (7.0-9.0), high selectivity, and low cyto-toxicity. Furthermore, IDPD-ONOO achieved the pulmonary intracellular imaging of both the exogenous and endogenous ONOO- level, which was meaningful for the research on the pulmonary physiological and pathological mechanism with optimized optical implements.
Published Version
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