Abstract
Objective To explore the necessity and feasibility of RBC blood typing by molecular technique for unidentified blood group. Methods The blood group was identified by serological test and molecular detection. Results One case of blood donor showed weakly positive reaction to anti -A, strongly positive reaction to anti-B and anti -H, weakly positive reaction to A 1 RBCs but no reaction to either B or O blood type. The sample was subjected to the detection of ABO genes and the results showed 9 mutants in Exons 6 and 7. The type B(A)02 was identified when comp a red with A101 allele. Conclusion Genetic detection is helpful for finding B(A) subgroup blood that cannot be identified by serological test.
Published Version
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