Abstract
Multiple sequence alignment (MSA) is one of the oldest dilemmas in computational biology, involving the alignment of more than two DNA, RNA, or protein sequences. There are numerous methods for aligning multiple sequences. According to the survey, there are 103 free MSA Tools – Software, and Resources available. Many techniques are designed to maximize speed while ignoring alignment accuracy. Similarly, many techniques prioritize accuracy over speed. The MSA tool's goal is to reduce execution time and memory requirements while increasing the accuracy of MSA on large-scale data. Choosing which tools to use is one of the most difficult problems for bioinformaticians, especially for the majority of non-bioinformaticians. As a result, a comparison study of the various multiple sequence alignment tools is required so that biologists and bioinformaticians can correctly interpret their biological data using the appropriate tools. In order to determine which tool is best for multiple sequence alignment, we used a case study to evaluate various MSA tools.
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